Research Groups

Mari group

Autophagy is an intracellular  process that allows the degradation and recycling of cellular components. This pathway is associated with numerous physiological functions and pathological conditions. Understanding its molecular principles is thus of primary relevance for medical applications.

  • People
  • Publications
  • Alumni
  • Biosketch
  • Research
  • Covers
  • Students
  • Muriel Mari Visit
    Position

    Assistant Professor, Principal Investigator

    Research fields

    Ultrastructural characterization of the process of autophagy

      • Selected Publications:
      1. Lassen KG, McKenzie CI, Mari M, Murano T, Begun J, Baxt LA, Goel G, Villablanca EJ, Kuo S.-Y., Huang H, Macia L, Bhan A, Batten M, Daly MJ, Reggiori F, Mackay CR, Xavier RK (2016). Genetic coding variant in GPR65 alters lysosomal pH and links lysosomal dysfunction with colitis risk. Immunity.  Jun 21;44(6):1392-405.  (pdf)
      2. Khaminets A, Heinrich T, Mari M, Grumati P, Huebner AK, Akutsu M, Liebmann L, Stolz A, Nietzsche S, Koch N, Mauthe M, Katon I, Qualmann B, Weis J, Reggiori F, Kurth I, Hübner CA, Dikic I (2015). Regulation of endoplasmic reticulum homeostasis by FAM134B-mediated selective autophagy. Nature, 522, 354-358. (pdf)
      3. Mari M, Geerts WJ, Reggiori F (2014). Immuno- and correlative light microscopy-electron tomography methods for 3D protein localization in yeast. Traffic, 15, 1164-78.  (pdf)
      4. Mari M, Griffith J, Reggiori F (2014). Nanogold labeling of the yeast endosomal system for ultrastructural analyses. J Vis Exp. (pdf)
      5. Hönscher C, Mari M, Auffarth K, Bohnert M, Griffith J, Geerts W, van der Laan M, Cabrera M, Reggiori F, Ungermann C (2014), Cellular metabolism regulates contact sites of vacuoles with mitochondria. Dev Cell, 30, 86-94.  (pdf)
      6. Nair U, Mari M, Baba M, Yen WL, Xie Z, Cao Y, Reggiori F, Klionsky DJ (2012). A role for Atg8-PE deconjugation in autophagosome biogenesis Autophagy, 8, 780-93.  (pdf)
      7. Mari M, Griffith J, Rieter E, Krishnappa L, Klionsky DJ, Reggiori F (2010). An Atg9-containing compartment that functions in the early steps of autophagosome biogenesis. J Cell Biol, 190, 1005-1022. (pdf)
      8. Griffith J, Mari M, De Mazière A, Reggiori F (2008). A cryosectioning procedure for the ultrastructural analysis and the immunogold labelling of yeast S. Cerevisiae. Traffic, 9, 1060-1072.
      9. Mari M, Reggiori F (2007). Review/opinion: Shaping membranes into autophagosomes. Nat Cell Biol, 10, 1125-7. (pdf)
      10. Mari M, Bujny MV, Zeuschner D, Geerts WJ, Griffith J, Petersen CM, Cullen PJ, Klumperman J, Geuze HJ (2008). SNX1 defines an early endosomal recycling exit for sortilin and mannose 6-phosphate receptors. Traffic, 3, 380-93.  (pdf)
  • Postdoctoral fellow
    Thomas Wassner (2006, visiting Postdoctoral fellow)

    PhD students

    Leticia Lemus (2015, visiting PhD student)
    Ann-Katrin Unger (2013, visiting PhD student)

    Master students
    Stephanie Kappes (2010–2011)
    Ester Rieter (2007-2008)
    Laurent Yvan-Charvet (2000)

    Technician trainees
    Kerst-Jan Hijlkema (2015-2016)
    Marinke van Oooschot (2009-2010)

  • Muriel Mari studied Biochemistry and Cell Biology at the University of Nice Sophia-Antipolis, France. In 2002, she obtained my PhD in Cell Biology and Physiology from the same Institution, in the laboratory of Dr. Yannick Lemarchand-Brustel. The research topic was the identification and characterization of new Rab4 effectors and their function in glucose transport. In 2002, she extended her studies in this area by joining as a postdoctoral fellow the laboratory of Prof. David James at the Garvan Institute in Sydney, Australia. There, sheinvestigated the membrane association of the SNARE-regulating protein Munc18c.

    In 2003 Mari joined the Department of Cell Biology at the University Medical Center Utrecht (The Netherlands) to acquire competencies in advanced electron microscopy methods. There, as a Marie-Curie postdoctoral fellow in the laboratory of Prof. Hans Geuze, she investigated the intracellular trafficking of lysosomal enzyme receptors in mammalian cells.

    In 2006, Mari joined  the group of Prof. Fulvio Reggiori for an additional postdoctoral experience in Utrecht and started working on the molecular mechanism of autophagy using yeast as a model system.

    In 2015,  Muriel Mari was appointed as an Assistant Professor at the Department of Cell Biology of the University Medical Center Groningen. She is currently studying autophagosome biogenesis in yeast and mammalian cells at the ultrastructural level using techniques such as high-pressure freezing/freeze substitution, electron microscopy, immuno-electron microscopy, electron tomography and correlative light and electron microscopy.

  • Our research is subdivided in two major areas of interest:

    1) Investigation of the membrane rearrangements underlying the biogenesis of the autophagosomes, the vesicular carriers that are the hallmark of autophagy. For our studies, we are mostly using yeast, Saccharomyces cerevisiae, as a model system. Studies are performed using immuno-electron microscopy, tomography and correlative electron and light microscopy methods, that we have adapted to explore the ultrastructure of this organism.

    3D-reconstruction of an autophagosomal intermediate using immuno-electron tomography. Left panel. Reconstruction of an autophagosomal intermediate obtained from a tomogram, superposed to one of the tomogram images. Right panel. The same structure observed from a different angle. Grey and green circular structures, cargoes; blue, phagophore; yellow, endoplasmic reticulum; red, mitochondrion; dark grey, vacuole.

    3D-reconstruction of an autophagosomal intermediate using immuno-electron tomography. Left panel. Reconstruction of an autophagosomal intermediate obtained from a tomogram, superposed to one of the tomogram images. Right panel. The same structure observed from a different angle. Grey and green circular structures, cargoes; blue, phagophore; yellow, endoplasmic reticulum; red, mitochondrion; dark grey, vacuole.

    2) Improvement and development of new electron microscopy approaches to increase the number of biological questions that can be addressed with these techniques.

    Our expertise in electron microscopy approaches is giving us the opportunity to  have numerous collaborations on studies multiple cell biological topics and in various organisms.

  • cover MIC2015Microb Cell (2015) Vol. 2. cover EMBO J 2015EMBO J. (2015)
    cover Methods 2015Methods. (2015) Vol. 75. cover traffic 2008Traffic (2008) Vol. 9.
    cover Traffic 2014

    Traffic (2014) Vol. 15.

    cover JCB 2010J Cell Biol (2010) Vol.190.

     

     

     

     

     

  • Student projects:

    Though there is currently not a defined research project, Masters students, from the different Master Programs at the University of Groningen, and technician trainees, from the different “Hogeschools” in the Netherlands, are welcome to contact Dr. Muriel Mari to discuss further internship possibilities.

    You can apply via this application form.

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