Autophagy is an intracellular process that allows the degradation and recycling of cellular components. This pathway is associated with numerous physiological functions and pathological conditions. Understanding its molecular principles is thus of primary relevance for medical applications.
Thomas Wassner (2006, visiting Postdoctoral fellow)
Leticia Lemus (2015, visiting PhD student)
Ann-Katrin Unger (2013, visiting PhD student)
Stephanie Kappes (2010–2011)
Ester Rieter (2007-2008)
Laurent Yvan-Charvet (2000)
Kerst-Jan Hijlkema (2015-2016)
Marinke van Oooschot (2009-2010)
Muriel Mari studied Biochemistry and Cell Biology at the University of Nice Sophia-Antipolis, France. In 2002, she obtained my PhD in Cell Biology and Physiology from the same Institution, in the laboratory of Dr. Yannick Lemarchand-Brustel. The research topic was the identification and characterization of new Rab4 effectors and their function in glucose transport. In 2002, she extended her studies in this area by joining as a postdoctoral fellow the laboratory of Prof. David James at the Garvan Institute in Sydney, Australia. There, sheinvestigated the membrane association of the SNARE-regulating protein Munc18c.
In 2003 Mari joined the Department of Cell Biology at the University Medical Center Utrecht (The Netherlands) to acquire competencies in advanced electron microscopy methods. There, as a Marie-Curie postdoctoral fellow in the laboratory of Prof. Hans Geuze, she investigated the intracellular trafficking of lysosomal enzyme receptors in mammalian cells.
In 2006, Mari joined the group of Prof. Fulvio Reggiori for an additional postdoctoral experience in Utrecht and started working on the molecular mechanism of autophagy using yeast as a model system.
In 2015, Muriel Mari was appointed as an Assistant Professor at the Department of Cell Biology of the University Medical Center Groningen. She is currently studying autophagosome biogenesis in yeast and mammalian cells at the ultrastructural level using techniques such as high-pressure freezing/freeze substitution, electron microscopy, immuno-electron microscopy, electron tomography and correlative light and electron microscopy.
Our research is subdivided in two major areas of interest:
1) Investigation of the membrane rearrangements underlying the biogenesis of the autophagosomes, the vesicular carriers that are the hallmark of autophagy. For our studies, we are mostly using yeast, Saccharomyces cerevisiae, as a model system. Studies are performed using immuno-electron microscopy, tomography and correlative electron and light microscopy methods, that we have adapted to explore the ultrastructure of this organism.
2) Improvement and development of new electron microscopy approaches to increase the number of biological questions that can be addressed with these techniques.
Our expertise in electron microscopy approaches is giving us the opportunity to have numerous collaborations on studies multiple cell biological topics and in various organisms.
|Microb Cell (2015) Vol. 2.||EMBO J. (2015)|
|Methods. (2015) Vol. 75.||Traffic (2008) Vol. 9.|
Traffic (2014) Vol. 15.
|J Cell Biol (2010) Vol.190.|
Though there is currently not a defined research project, Masters students, from the different Master Programs at the University of Groningen, and technician trainees, from the different “Hogeschools” in the Netherlands, are welcome to contact Dr. Muriel Mari to discuss further internship possibilities.
You can apply via this application form.