||Associate Professor, Principal Investigator
||Membrane domains, sphingolipids and ABC transporters
- Research Profile
- Selected Publications
Jan Willem Kok has a longstanding interest in sphingolipids and membrane cell biology, particularly lipid-protein interactions in membrane domains, and including membrane dynamics, trafficking and transport.
He obtained his PhD on ‘Lipid traffic in animal cells’ in 1991. He went on as Research fellow for the Royal Netherlands Academy of Arts and Sciences to combine the fields of lipid traffic and bioactivity of sphingolipids in the context of defence strategies of tumour cells.
As an assistant- (1998) and later associate Professor (2005) he focussed on the role of the membrane environment, lipid rafts and the actin cytoskeleton in ABC transporter modulation and multidrug resistance of tumour cells. Later he applied his expertise on membranes and ABC transporters to the field of astrocyte biology.
- Younes, J.A., K. Klappe, J.W. Kok, H.J. Busscher, G. Reid G, and H.C. van der Mei. 2016. Vaginal epithelial cells regulate membrane adhesiveness to co-ordinate bacterial adhesion. Cell Microbiol. 18, 605-614. (pdf)
- Hummel, I., K. Klappe, C. Ercan, and J.W. Kok. 2011. MRP1 function and localization depend on cortical actin. Mol. Pharm. 79, 229-240. (pdf)
- Klappe, K., A.J. Dijkhuis, I. Hummel, A. van Dam, P.T. Ivanova, S.B. Milne, D.S. Myers, H.A. Brown, H. Permentier, and J.W Kok. 2010. Extensive sphingolipid depletion does not affect lipid raft integrity or lipid raft localization and efflux function of the ABC transporter MRP1. Biochem. J. 430, 519-29. (pdf)
- Dijkhuis, A.J., K. Klappe, S. Jacobs, B.J. Kroesen, W. Kamps, H. Sietsma, and J.W. Kok. 2006. PDMP sensitizes neuroblastoma to paclitaxel by inducing aberrant cell cycle progression leading to hyperploidy. Mol. Cancer. Ther. 5, 593-601. (pdf)
- Hinrichs, J.W.J., K. Klappe, I. Hummel, and J.W. Kok. 2004. ATP-binding cassette transporters are enriched in non-caveolar detergent-insoluble glycosphingolipid-enriched membrane domains (DIGs) in human multidrug-resistant cancer cells. J. Biol. Chem. 279, 5734-5738. (pdf)
- Veldman, R.J., K. Klappe, J. Hinrichs, I. Hummel, G. van der Schaaf, H. Sietsma, and J.W. Kok. 2002. Altered sphingolipid metabolism in multidrug resistant ovarian cancer cells is due to uncoupling of glycolipid biosynthesis in the Golgi apparatus. FASEB J. 10.1096/fj.01-0863fje. (pdf)
- Sietsma, H., R.J. Veldman, B. Ausema, W. Nijhof, W. Kamps, E. Vellenga, and J.W. Kok. 2000. 1-phenyl-2-decanoylamino-3-morpholino-1-propanol chemosensitizes neuroblastoma cells for taxol and vincristine. Clin. Cancer Res. 6, 942-948. (pdf)
- Sietsma, H., W. Nijhof, B. Dontje, E. Vellenga, W. Kamps, and J. W. Kok. 1998. Inhibition of hemopoiesis in vitro by neuroblastoma-derived gangliosides. Cancer Res. 58, 4840-4844. (pdf)
- Kok, J.W., T. Babia, G. Egea and D. Hoekstra. 1998. PDMP blocks brefeldin A-induced retrograde membrane transport from Golgi to ER; Evidence for involvement of calcium homeostasis and dissociation from sphingolipid metabolism. J. Cell Biol. 142, 25-38. (pdf)
- Kok, J.W., M. Nikolova-Karakashian, K. Klappe, C. Alexander and A.H. Merrill, Jr. 1997. Dihydroceramide biology. Structure specific metabolism and transport. J. Biol. Chem. 272, 21128-21136. (pdf)